Journal: PLoS ONE

Article Title: Clinical, pathological, and molecular features of classical and L-type atypical-BSE in goats

doi: 10.1371/journal.pone.0198037

Figure Lengend Snippet: Normalized rapid test results: IDEXX HerdChek® BSE-scrapie EIA Short and Ultra Short protocol–small ruminant conjugate, IDEXX HerdChek™ BSE- scrapie EIA Short and Ultra Short protocol—bovine conjugate; Bio-Rad TeSeE™ and Bio-Rad Sheep and Goat™.

Article Snippet: In order to compare the diagnostic sensitivity of EU-approved rapid tests, medulla oblongata samples from 4 C-BSE, 5 L-BSE experimentally infected goats and 2 samples from control animals were tested in parallel with IDEXX HerdChek® BSE-scrapie EIA (HerdChek BSE-scrapie antigen test kit, EIA, IDEXX Laboratories, Westbrook, ME, USA) Short and Ultra Short protocol–small ruminant conjugate, IDEXX HerdChek® BSE-scrapie EIA Short and Ultra Short protocol—bovine conjugate; Bio-Rad TeSeETM SAP (TeSeE SAP purification-detection test kit, Bio-Rad Laboratories, Marnes-La-Coquette, France) and Bio-Rad TeSeETM Sheep and GoatTM (TeSeE sheep & goat purification-detection test kit, Bio-Rad Laboratories).

Techniques: Control

Journal: PLoS ONE

Article Title: Emergence of Classical BSE Strain Properties during Serial Passages of H-BSE in Wild-Type Mice

doi: 10.1371/journal.pone.0015839

Figure Lengend Snippet: Mice inoculated with with H-BSE (lanes 1–3 and 5–7 from isolates 01-2604 and 03-2095 respectively), at first (lanes 1, 5) or second passage (lanes 2–3 and 6–7), were compared to mice infected with classical BSE (lanes 4, 8) in panels A–C. At second passage, PrP res exhibited either H-type features, from mice that died later (491 and 504 d.p.i. in lanes 2 and 6 respectively), or “C-BSE like” features (464 and 322 d.p.i. in lanes 3 and 7 respectively). The brain tissue equivalents loaded per lane are indicated (in tenth of mg). D - Glycoform proportions (means +/− standard deviations) of PrP res from H-BSE (isolate 03-2095)-infected mice detected using Sha31 antibody. Mice exhibited PrP res with a molecular mass either similar to that of H-BSE (full squares) or to classical BSE (full diamonds). E - PrP d extracted in the absence of protease digestion and deglycosylated by PNGase treatment from H-BSE infected mice at first (lane 1) or second passage (lanes 2-3 from mice that died at 497 d.p.i. and 464 d.p.i. respectively), compared to a classical BSE control in lane 4 (0.3 mg brain tissue equivalent per lane). Full-length PrP d (FL) and C1 and C2 fragments are indicated. Panels A and B were revealed by monoclonal antibodies Sha31 and 12B2 respectively and panels C and E by HRP-labelled SAF84 antibody. Bars to the left indicate the 29.0, 20.1 and 14.3 kDa marker positions.

Article Snippet: PrP res or PrP d were detected using the monoclonal antibodies anti-PrP Sha31 (1/10 from kit TeSeE sheep/goat Biorad), SAF84 (500 ng/ml)(SPI-Bio, France) or 12B2 (340 ng/ml) against the 144-WEDRYYRE-151, 163-RPVDQY-168 and 88-WGQGG-92 murine PrP sequences respectively.

Techniques: Infection, Control, Bioprocessing, Marker

Journal: PLoS ONE

Article Title: Emergence of Classical BSE Strain Properties during Serial Passages of H-BSE in Wild-Type Mice

doi: 10.1371/journal.pone.0015839

Figure Lengend Snippet: A - Detection of PrP res from spleens of mice inoculated with H-BSE isolates 01-2604 (lanes 1–3) or 03-2095 (lanes 4–9) at first passage. B - Detection of PrP res from spleens of mice inoculated with H-BSE at third passage in mice inoculated with a brain homogenate with “C-BSE like” PrP res (lanes 4–8). PrP res from the brain of one of these mice (lanes 3, 9) and from brains of C506M3 scrapie (lanes 1, 11) or classical BSE (lanes 2, 10) controls are shown for comparison. C - PrP res from spleens of mice infected with H-BSE at first passage (lanes 2, 4)(isolates 03-1928 and 03-2095), second passage in a mouse with “C-BSE like” PrP res (lane 6) and third passage from mice inoculated with a brain homogenate with “C-BSE like” PrP res (lanes 8, 10). PrP res from spleens of classical BSE (lanes 3, 5, 7, 9) and C506M3 (lanes 1, 11) controls are shown. Panels A and B were revealed by monoclonal antibody Sha31 and panel C by HRP-labelled SAF84 antibody Bars to the left indicate the 29.0 and 20.1 kDa marker positions in panels A and B or the 20.1 and 14.3 kDa marker positions in panels C and D.

Article Snippet: PrP res or PrP d were detected using the monoclonal antibodies anti-PrP Sha31 (1/10 from kit TeSeE sheep/goat Biorad), SAF84 (500 ng/ml)(SPI-Bio, France) or 12B2 (340 ng/ml) against the 144-WEDRYYRE-151, 163-RPVDQY-168 and 88-WGQGG-92 murine PrP sequences respectively.

Techniques: Comparison, Infection, Marker